Safety of Wet-Primed Cardiopulmonary Bypass Circuits during prolonged standby
- #PER 01-O-8
- Perfusion. SESSION-1
- Oral
Safety of Wet-Primed Cardiopulmonary Bypass Circuits during prolonged standby
Umpaipan Thana, TANYONG PIPANMEKAPORN, THITIPONG TEPSUWAN, JARIN PHIROMPAKSAR, NIRAMOL BALCHON
CHIANG MAI UNIVERSITY, CHIANG MAI, Thailand
Date, time and location: 2018.05.26 15:30, Press Hall, 2F
Abstract
Background: The immediate establishment of cardiopulmonary
bypass (CPB) is a critical step in emergency cardiac surgery. A pre-assembled
CPB circuit is sometimes necessary in order to shorten the time. Only a few
studies have tested the sterility of CPB circuits in a clinical environment.
This lack of evidence means many cardiac surgeons are still reluctant to use them.
The purpose of this study is to determine safety periods regarding sterility in
wet-primed CPB circuits under normal conditions in an operating room
environment.
Method: Experimental CPB circuits are assembled under routine
clinical conditions and primed with 2000 ml of Acetate Ringer’s solution. The
sterility of 9 pre-assembled CPB circuits were studied for 168 h. Three
circuits were assembled and stored uncovered in one corner of the OR which was
used for routine cardiac surgery. Six
circuits were assembled and stored in the perfusionist office. They were left
unattended and were uncovered. The priming solution was continuously
recirculated in the experimental CPB circuits. Fifty ml of circulating fluid
were collected at the sampling port under aseptic conditions in order to test
for microbial growth every 0, 24, 48, 72, 96, 120, 144 and 168 h. The membrane
filter method was used to identify any microbial growth.
Result: Microbiology of all samples from both groups were
negative at every time period apart from the occurrence of Bacillus
species in two samples from the equipment held in the perfusionist office at 96
h. However they were considered as contamination as further samples (after 96 h) were negative
Conclusion: The sterility of wet-primed CPB circuits could be
maintained for up to 168 h. The strategy to have primed circuits available
could be beneficial in emergency situations without extra expense.